Features: Alkaline phosphatase enzyme detection system, Avidin/biotin technology, High sensitivity/low background, Low cost Preferred for tissues that have high endogenous peroxidase activity, Offers additional substrate choices, Used as a second label in multiple antigen labeling applications.
Available without ("Standard kit) or with biotinylated species-specific antibody.
Kit Contents: 3 ml Normal Goat Serum, 1 ml Biotinylated Goat Anti-Rabbit IgG, 2 ml Reagent A (Avidin DH) plus 2 ml Reagent B (Biotinylated AP).
With more than 50,000 citations to its credit, the VECTASTAIN ABC kit remains widely popular. Based on the versatile avidin-biotin complex interaction, the system is modular, and along with our selection of secondary antibodies, can accommodate a wide array of primary antibody and tissue species. Our ABC kits are economical and continue to be a staple product in any immunohistochemistry (IHC) and immunocytochemistry (ICC) laboratory.
The sensitivity of the VECTASTAIN ABC-AP system is comparable to that of the peroxidase VECTASTAIN Elite® ABC system. The VECTASTAIN ABC-AP Kits may be preferred for tissues that have high endogenous peroxidase activity. The system also offers additional substrate color choices.
These kits are sufficient to stain approximately 1000-2000 sections or fifty 100 cm2 blots.