Monosan Blockingsolution - SuperBlock

Intended use: Blocking Solution is developed to eliminate unspecific binding of primary and secondary antibodies to tissue sections. It is primarily intended to be used in immunohistochemistry on formalin-fixed paraffin-embedded samples.

Summary and explanation: Unspecific binding of primary and secondary antibodies to tissue sections in immunohistochemical staining procedures can result in background staining. This effect can be eliminated when tissue sections are incubated with Blocking Solution prior to incubation with the primary antibody. The protein in Blocking Solution abolishes unspecific binding. Blocking Solution is a universal blocking reagent. Unlike other frequently used blocking solutions (e. g. serum blocks) the reagent can be used regardless of the origin species of the secondary antibody. Interferences with secondary antibodies or other components of detection systems are not observed. In contrast to other blocking reagents this Blocking Solution should not be incubated longer than 10 minutes and should be rinsed away with wash buffer. Otherwise the signal intensity of the immunohistochemical staining reaction could be decreased.

Reagents provided: 100 ml Blocking Solution (ready-to-use)

Storage and handling: The solution should be stored at 2-8°C without further dilution. Please store the reagent in a dark place and do not freeze it. Under these conditions the solution is stable up to the expiry date indicated on the label. Do not use product after the expiry date. A positive and a negative control have to be carried out in parallel to the test material. If you observe unusual staining or other deviations from the expected results which could possibly be caused by this reagent, please contact our technical support.

Principle of method: Blocking Solution is applied on tissue sections to reduce background staining due to unspecific binding of primary and secondary antibodies in immunohistochemistry. The step is carried out prior to incubation with the primary antibody

Procedure: 1. Apply Blocking Solution for 5 minutes at room temperature. The section should be covered completely. 2. Rinse with wash buffer. 3. Proceed with next steps for immunohistochemical staining as usual starting with the primary antibody.

Expected results: During the reaction of the substrate with horse radish peroxidase or alkaline phosphatase in the presence of a chromogen, a coloured precipitate is formed at the location of the bound primary antibody. This reaction only takes place if the target antigen is existent in the tissue. The chromogen used determines the colour of the precipitate. The analysis is carried out using a light microscope.

References: Elias JM Immunohistopathology – A practical Approach to Diagnosis ASCP Press 2003/Nadji M and Morales AR Ann N.Y. Acad Sci 420:134-139, 1983

• Artikelnummer: SANMON-APP144
• Hersteller: Sanbio / Monosan
• Hersteller Artikelnummer: MON-APP144
• Verpackungseinheit: 100 ml
• Mengeneinheit: STK
• Methode: Immunofluorescence, Immunohistochemistry (frozen), Immunohistochemistry (paraffin)