Abbreviation: 25-HVD3
Target Species: Universal
Usage: This ELISA kit applies to the in vitro quantitative determination of 25-HVD3 concentrations in serum, plasma and other biological fluids.
Tested Sample Types: serum, plasma and other biological fluids
Assay Time: 2.5h
Sensitivity: 1.88 ng/mL
Detection Range: 3.13-200 ng/mL
Test Principle: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal 25-HVD3. During the reaction, Universal 25-HVD3 in the sample or standard competes with a fixed amount of Universal 25-HVD3 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal 25-HVD3. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal 25-HVD3 in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Cross reactivity: This kit recognizes Universal 25-HVD3 in samples.No significant cross-reactivity or interference between Universal 25-HVD3 and analogues was observed