Chemi-Verse™ PDGFRα (D842I) Kinase Assay Kit

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Background: PDGFRα (platelet-derived growth factor receptor A), also known as CD140a, is one of the isoforms of the tyrosine kinase receptor for PDGF (platelet-derived growth factor), belonging to the family of RTK class III family of proteins. Binding of PDGF induces receptor dimerization and trans-phosphorylation, which leads to the recruitment of signaling and adaptor proteins and activation of downstream signaling cascades, such as the PI3K (phosphoinositide 3-kinase)-mTOR (mammalian target of rapamycin). It has been implicated in regulation of cell growth and survival, apoptosis, and differentiation. It has been identified as a potential target in eosinophilic leukemia cancer, inflammatory breast cancer, and gastrointestinal stromal tumors (GIST). GIST is one of the most common mesenchymal cancers in the gastrointestinal tract, with 10-15% of the mutations leading to constitutively active PDGFRα. The D842I is a mutation in exon 18, being imatinib resistant. The study of PDGFRα and the development of new inhibitors may benefit the cancer therapy field.

Contraindications: The final concentration of DMSO in the assay should not exceed 1%.

Description: The Chemi-Verse™ PDGFRα (D842I) Kinase Assay Kit is designed to measure PDGFRα (platelet-derived growth factor receptor A) (D842I) tyrosine kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified PDGFRα (D842I) (amino acids 550-1089(end)), kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.

Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed. 

Supplied As: The assay kit comes in a convenient 96-well format, with enough purified PDGFRα (D842I) (amino acids 550-1089(end)), kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.

Uniprot: P16234

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: Guerit E., et al., 2021 Cell Mol Life Sci 78(8):3867-3881.Sun Y., et al., 2022 Front Oncol. 12: 927587.

• Artikelnummer: BPS82752
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 82752
• Verpackungseinheit: 96 reactions
• Mengeneinheit: PAK