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One-step TUNEL Flow Cytometry Apoptosis Kit (Red, Elab Fluor 594)
One-step TUNEL Flow Cytometry Apoptosis Kit (Red, Elab Fluor 594)
Artikelnummer
ELSE-CK-A422-20
Verpackungseinheit
20 Assays
Hersteller
Elabscience Biotechnology
Verfügbarkeit:
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Assay Time: 2.5-3 hours
Test Principle: When cells undergo apoptosis, specific DNA endonucleases will be activated, cutting the genomic DNA between the nucleosomes. The DNA of apoptotic cells is cleaved into multimers of 180~200bp fragments, corresponding to the oligonucleosomal size. Therefore, the DNA of apoptotic cells typically migrates as a ladder of 180~200bp on an agarose gel. The exposed 3'-OH of the broken DNA can be catalyzed by Terminal Deoxynucleotidyl Transferase (TdT) with fluorescein labeled dUTP, which can be detected with flow cytometry.
Test Principle: When cells undergo apoptosis, specific DNA endonucleases will be activated, cutting the genomic DNA between the nucleosomes. The DNA of apoptotic cells is cleaved into multimers of 180~200bp fragments, corresponding to the oligonucleosomal size. Therefore, the DNA of apoptotic cells typically migrates as a ladder of 180~200bp on an agarose gel. The exposed 3'-OH of the broken DNA can be catalyzed by Terminal Deoxynucleotidyl Transferase (TdT) with fluorescein labeled dUTP, which can be detected with flow cytometry.
| Artikelnummer | ELSE-CK-A422-20 |
|---|---|
| Hersteller | Elabscience Biotechnology |
| Hersteller Artikelnummer | ELA-E-CK-A422-20 |
| Green Labware | Nein |
| Verpackungseinheit | 20 Assays |
| Mengeneinheit | PAK |
| Produktinformation (PDF) | Download |
| MSDS (PDF) |
|
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Gernot Ensinger, M.Sc.
Laurent Haze
