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3-NT(3-Nitrotyrosine) ELISA Kit
3-NT(3-Nitrotyrosine) ELISA Kit
Artikelnummer
ELSE-EL-0040-48
Verpackungseinheit
48 T
Hersteller
Elabscience Biotechnology
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Abbreviation: 3-NT
Target Synonym: 3-Nitro-L-Tyrosine;Nitrotyrosine;
Target Species: Universal
Usage: This ELISA kit applies to the in vitro quantitative determination of 3-NT concentrations in serum, plasma and other biological fluids.
Tested Sample Types: serum, plasma and other biological fluids
Assay Time: 2.5h
Sensitivity: 0.94 ng/mL
Detection Range: 1.56-100 ng/mL
Test Principle: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal 3-NT. During the reaction, Universal 3-NT in the sample or standard competes with a fixed amount of Universal 3-NT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal 3-NT. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal 3-NT in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Cross reactivity: This kit recognizes Universal 3-NT in samples.No significant cross-reactivity or interference between Universal 3-NT and analogues was observed
Target Synonym: 3-Nitro-L-Tyrosine;Nitrotyrosine;
Target Species: Universal
Usage: This ELISA kit applies to the in vitro quantitative determination of 3-NT concentrations in serum, plasma and other biological fluids.
Tested Sample Types: serum, plasma and other biological fluids
Assay Time: 2.5h
Sensitivity: 0.94 ng/mL
Detection Range: 1.56-100 ng/mL
Test Principle: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal 3-NT. During the reaction, Universal 3-NT in the sample or standard competes with a fixed amount of Universal 3-NT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal 3-NT. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal 3-NT in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Cross reactivity: This kit recognizes Universal 3-NT in samples.No significant cross-reactivity or interference between Universal 3-NT and analogues was observed
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