8-epi-PGF2alpha(8-Epi-Prostaglandin F2 Alpha) ELISA Kit

Abbreviation: 8-epi-PGF2α

Target Synonym: 15-F2t-IsoP;8-iso-PGF2a;8-isoprostane;8-iso-PG;8-iso Prostaglandin;

Target Species: Universal

Usage: This ELISA kit applies to the in vitro quantitative determination of 8-epi-PGF2α concentrations in serum, plasma and other biological fluids.

Tested Sample Types: serum, plasma and other biological fluids

Assay Time: 2.5h

Sensitivity: 9.38 pg/mL

Detection Range: 15.63-1000 pg/mL

Test Principle: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal 8-epi-PGF2α. During the reaction, Universal 8-epi-PGF2α in the sample or standard competes with a fixed amount of Universal 8-epi-PGF2α on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal 8-epi-PGF2α. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal 8-epi-PGF2α in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Cross reactivity: This kit recognizes Universal 8-epi-PGF2α in samples.No significant cross-reactivity or interference between Universal 8-epi-PGF2α and analogues was observed

• Artikelnummer: ELSE-EL-0041-48
• Hersteller: Elabscience Biotechnology
• Hersteller Artikelnummer: ELA-E-EL-0041-48
• Green Labware: Nein
• Verpackungseinheit: 48 T
• Mengeneinheit: PAK
• Methode: ELISA