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Applications: Useful for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Background: AKT1, also known as Protein Kinase B Alpha (PKBα), is a serine/threonine kinase found in various cell types throughout the body, where it plays a critical role in regulating cell survival, insulin signaling, angiogenesis and tumor formation as well as controlling apoptosis. Importantly, the signaling pathway involving AKT1 kinase appears to be essential for the normal development and function of the nervous system. Dysregulation of Akt1 contributes to a number of serious diseases, including cancer, diabetes, cardiovascular and neurological diseases.
Description: The AKT1 Assay Kit is designed to measure AKT1 activity for screening and profiling applications using Kinase-Glo® Kinase Assay as a detection reagent.
Storage Stability: Up to 6 months when stored as recommended.
Supplied As: The AKT1 Assay Kit comes in a convenient 96-well format, with enough purified recombinant AKT1 enzyme, AKT1 Substrate, ATP, and kinase assay buffer for 100 enzyme reactions.
Biosafety Level: Not applicable (BSL-1)