Chemi-Verse™ Wee1 Kinase Assay Kit

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Application: Study enzyme kinetics and screen small molecule inhibitors for drug discovery and high throughput screening (HTS) applications.

Background: Wee1 (also known as WEE1 G2 checkpoint kinase) belongs to a dual kinase (tyrosine and serine-threonine kinase) family of proteins that includes PKMYT1 (membrane-associated tyrosine and threonine specific cdc2-inhibitory kinase), Wee1 and Wee1B. Wee1 plays a crucial role in regulating G2/M transition and S-phase through phosphorylation of cyclin dependent kinase (CDKs), allowing DNA damage to be repaired, and it is thus found in the nucleus of cells. It can phosphorylate tyrosine residues, possibly due to its evolution from a serine-threonine kinase by mutation. Its activity is supported by proteins like 14-3-3 proteins or Hsp90 (heat-shock protein 90) that enhance its stability and activity. Wee1 is downregulated in many cancer types, indicating it has a tumor suppressor role. However, it has also been reported that some tumors overexpress Wee1, such as glioma and HCC (hepatocellular carcinoma). Classical cancer therapies, such as chemotherapy and radiotherapy, induce DNA damage, and cells trigger the checkpoint in cell cycle, such as Wee1. This can lead to cancer cell resistance. Inhibiting Wee1 prevents cells from repairing DNA damage due to unchecked replication, suggesting that combination therapeutics with DNA damaging reagents and Wee1 inhibitors may be effective against cancer.

Contraindications: The final concentration of DMSO in the assay should not exceed 1%.

Description: The Chemi-Verse™ Wee1 Kinase Assay Kit is designed to measure Wee1 serine/threonine kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified Wee1 (amino acids 215-646(end)), kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.

Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.

Uniprot: P30291

Warnings: Avoid freeze/thaw cycles

Biosafety Level: Not applicable (BSL-1)

References: Esposito F., et al., 2021 Int J Mol Sci 22(19):10689.

• Artikelnummer: BPS82599
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 82599
• Verpackungseinheit: 96 reactions
• Mengeneinheit: PAK