DR3:TL1A Inhibitor Screening Assay Kit-384 reactions

Products from BPS Bioscience require a minimum order value above 400€

Applications: Screen or titrate small molecule inhibitors or biologics for drug discovery and high-throughput screening (HTS) applications of the TL1A binding to DR3.

Background: DR3 (death receptor 3), also known as tumor necrosis factor receptor superfamily member 25 or TNFRSF25, is a membrane receptor of the tumor necrosis factor receptor superfamily of proteins (TNFRSF), which associates with TL1A (TNF-like protein 1A) in T and NK cells. DR3 has been recognized as a significant anti-apoptotic and differentiation factor and it is a co-stimulatory receptor. TL1A, also called TNFSF15, is a member of the tumor necrosis factor family. It is expressed in different immune cells, such as monocyte, macrophage, dendritic cell, T cell and non-immune cells. TL1A competitively binds to DR3, having a higher affinity for DcR3 (decoy receptor 3), providing stimulatory signal for downstream signaling pathways. It then regulates proliferation, activation, apoptosis, and chemokine production in effector cells. The role of DR3 in T cell activation, and consequently in cytokine secretion and cell proliferation, makes it an attractive target in cancer therapy. Inhibition of DR3-TL1A interaction has substantial therapeutic potential in the treatment of solid tumors.

Contraindications: The final concentration of DMSO in the assay should not exceed 1%. 

Description: The DR3:TL1A Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of the interaction between DR3 (Death Receptor 3) and TL1A (TNF-like ligand 1A). This kit comes in a convenient 96-well or 384-well format, with biotin-labeled TL1A, purified DR3, streptavidin-labeled HRP, and assay buffer for 100 or 400 binding reactions.

Format: 96 reactions

Catalog #	Name	Amount	Storage
101882	TL1A, His-Tag, Avi-Tag, Biotin-Labeled*	5 µg	-80 °C
	DR3*	25 µg	-80 °C
	5x PP-02 Buffer	4 ml	-20 °C
	Blocking Buffer 7	40 ml	+4 °C
79742	Streptavidin-HRP	10 µl	+4 °C
79670	ELISA ECL Substrate A (translucent bottle)	6 ml	Room Temp
	ELISA ECL Substrate B (brown bottle)	6 ml	Room Temp
79699	96-well white microplate	1	Room Temp

* The concentration of protein is lot-specific and will be indicated on the tube containing the protein. 384 reactions

Catalog #	Name	Amount	Storage
101882	TL1A, His-Tag, Avi-Tag, Biotin-Labeled*	2 x 5 µg	-80 °C
	DR3*	2 x 25 µg	-80 °C
	5x PP-02 Buffer	4 ml	-20 °C
	Blocking Buffer 7	2 x 40 ml	+4 °C
79742	Streptavidin-HRP	2 x 10 µl	+4 °C
79670	ELISA ECL Substrate A (translucent bottle)	2 x 6 ml	Room Temp
	ELISA ECL Substrate B (brown bottle)	2 x 6 ml	Room Temp
78188	384-well white microplate	1	Room Temp

* The concentration of protein is lot-specific and will be indicated on the tube containing the protein.

Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed

Uniprot: O95150

Warnings: Avoid freeze/thaw cyles

Biosafety Level: Not applicable (BSL-1)

References: Xu, W. D., et al., 2022 Front. Immunol. 13: 1-10.
Zwolak, A., et al., 2022 Sci. Rep. 12(1): 20538.

• Artikelnummer: BPS82241-2
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 82241-2
• Green Labware: Nein
• Verpackungseinheit: 384 reactions
• Mengeneinheit: PAK