Usage: This ELISA kit applies to the in vitro quantitative determination of Human D2D concentrations in plasma.
Tested Sample Types: plasma
Assay Time: 1.5h
Sensitivity: 53.1 pg/mL
Detection Range: 125-4000 pg/mL
Test Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human D2D. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human D2D are added to the micro ELISA plate wells. Human D2D in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human D2D.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value is proportional to the concentration of Human D2D. The concentration of Human D2D in the samples is then determined by comparing the OD of the samples to the standard curve.
Cross reactivity: This kit recognizes Human D2D in samples.No significant cross-reactivity or interference between Human D2D and analogues was observed