Human DHEA-S(Dehydroepiandrosterone Sulfate) ELISA Kit

Abbreviation: DHEA-S

Target Synonym: Androstenolone sulfate;Prasterone sulfate;Androst-5-en-3β-ol-17-one 3β-sulfate;

Target Species: Human

Usage: This ELISA kit applies to the in vitro quantitative determination of Human DHEA-S concentrations in Serum, plasma and other biological fluids.

Tested Sample Types: Serum, plasma and other biological fluids

Assay Time: 2.5h

Sensitivity: 9.38 ng/mL

Detection Range: 15.63-1000 ng/mL

Test Principle: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human DHEA-S. During the reaction, Human DHEA-S in the sample or standard competes with a fixed amount of Human DHEA-S on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human DHEA-S. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human DHEA-S in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Cross reactivity: This kit recognizes Human DHEA-S in samples.No significant cross-reactivity or interference between Human DHEA-S and analogues was observed

• Artikelnummer: ELSE-EL-H2019-48
• Hersteller: Elabscience Biotechnology
• Hersteller Artikelnummer: ELA-E-EL-H2019-48
• Green Labware: Nein
• Verpackungseinheit: 48 T
• Mengeneinheit: PAK
• Methode: ELISA