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Human GLP-2(Glucagon Like Peptide 2) ELISA Kit
Human GLP-2(Glucagon Like Peptide 2) ELISA Kit
Artikelnummer
ELSE-EL-H2238-48
Verpackungseinheit
48 T
Hersteller
Elabscience Biotechnology
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Abbreviation: GLP-2
Target Synonym: GLP2
Target Species: Human
Usage: This ELISA kit applies to the in vitro quantitative determination of Human GLP-2 concentrations in serum, plasma and other biological fluids.
Tested Sample Types: serum, plasma and other biological fluids
Assay Time: 2.5h
Sensitivity: 0.1 ng/mL
Detection Range: 0.16-10 ng/mL
UNIProt ID: P01275
Test Principle: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human GLP-2. During the reaction, Human GLP-2 in the sample or standard competes with a fixed amount of Human GLP-2 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human GLP-2. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human GLP-2 in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Cross reactivity: This kit recognizes Human GLP-2 in samples.No significant cross-reactivity or interference between Human GLP-2 and analogues was observed
Target Synonym: GLP2
Target Species: Human
Usage: This ELISA kit applies to the in vitro quantitative determination of Human GLP-2 concentrations in serum, plasma and other biological fluids.
Tested Sample Types: serum, plasma and other biological fluids
Assay Time: 2.5h
Sensitivity: 0.1 ng/mL
Detection Range: 0.16-10 ng/mL
UNIProt ID: P01275
Test Principle: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human GLP-2. During the reaction, Human GLP-2 in the sample or standard competes with a fixed amount of Human GLP-2 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human GLP-2. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human GLP-2 in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Cross reactivity: This kit recognizes Human GLP-2 in samples.No significant cross-reactivity or interference between Human GLP-2 and analogues was observed
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Gernot Ensinger, M.Sc.
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