English
Human IDE(Insulin Degrading Enzyme) ELISA Kit
Human IDE(Insulin Degrading Enzyme) ELISA Kit
Artikelnummer
ELSE-EL-H0429-48
Verpackungseinheit
48 T
Hersteller
Elabscience Biotechnology
Verfügbarkeit:
wird geladen...
Preis wird geladen...
Abbreviation: IDE
Target Synonym: Insulysin;Insulin protease;
Target Species: Human
Usage: This ELISA kit applies to the in vitro quantitative determination of Human IDE concentrations in serum, plasma and other biological fluids.
Tested Sample Types: serum, plasma and other biological fluids
Assay Time: 3.5h
Sensitivity: 37.5 pg/mL
Detection Range: 62.5-4000 pg/mL
UNIProt ID: P14735
Test Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IDE. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IDE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IDE, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IDE. You can calculate the concentration of Human IDE in the samples by comparing the OD of the samples to the standard curve.
Cross reactivity: This kit recognizes Human IDE in samples. No significant cross-reactivity or interference between Human IDE and analogues was observed
Target Synonym: Insulysin;Insulin protease;
Target Species: Human
Usage: This ELISA kit applies to the in vitro quantitative determination of Human IDE concentrations in serum, plasma and other biological fluids.
Tested Sample Types: serum, plasma and other biological fluids
Assay Time: 3.5h
Sensitivity: 37.5 pg/mL
Detection Range: 62.5-4000 pg/mL
UNIProt ID: P14735
Test Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IDE. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IDE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IDE, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IDE. You can calculate the concentration of Human IDE in the samples by comparing the OD of the samples to the standard curve.
Cross reactivity: This kit recognizes Human IDE in samples. No significant cross-reactivity or interference between Human IDE and analogues was observed
Verwandte Artikel
Markieren Sie die Artikel, um Sie dem Warenkorb hinzuzufügen oder
Immer für Sie da
Gernot Ensinger, M.Sc.
Laurent Haze
