LTB4(Leukotriene B4) ELISA Kit

Abbreviation: LTB4

Target Synonym: Leukotriene B4

Target Species: Universal

Usage: This ELISA kit applies to the in vitro quantitative determination of LTB4 concentrations in serum, plasma and other biological fluids.

Tested Sample Types: serum, plasma and other biological fluids

Assay Time: 2.5h

Sensitivity: 9.38 pg/mL

Detection Range: 15.63-1000 pg/mL

Test Principle: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal LTB4. During the reaction, Universal LTB4 in the sample or standard competes with a fixed amount of Universal LTB4 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal LTB4. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal LTB4 in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Cross reactivity: This kit recognizes Universal LTB4 in samples.No significant cross-reactivity or interference between Universal LTB4 and analogues was observed

• Artikelnummer: ELSE-EL-0061-48
• Hersteller: Elabscience Biotechnology
• Hersteller Artikelnummer: ELA-E-EL-0061-48
• Green Labware: Nein
• Verpackungseinheit: 48 T
• Mengeneinheit: PAK
• Methode: ELISA