Mouse ANP(Atrial Natriuretic Peptide) ELISA Kit

Abbreviation: ANP

Target Synonym: NPPA;ANF;ANP;ATFB6;ATRST2;CDD;CDD-ANF;CDP;PND;Atrial natriuretic peptide;natriuretic peptide A;

Target Species: Mouse

Usage: This ELISA kit applies to the in vitro quantitative determination of Mouse ANP concentrations in serum, plasma and other biological fluids.

Tested Sample Types: serum, plasma and other biological fluids

Assay Time: 2.5h

Sensitivity: 9.38 pg/mL

Detection Range: 15.63-1000 pg/mL

UNIProt ID: P05125

Test Principle: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Mouse ANP. During the reaction, Mouse ANP in the sample or standard competes with a fixed amount of Mouse ANP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Mouse ANP. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Mouse ANP in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Cross reactivity: This kit recognizes Mouse ANP in samples.No significant cross-reactivity or interference between Mouse ANP and analogues was observed

• Artikelnummer: ELSE-EL-M0166-48
• Hersteller: Elabscience Biotechnology
• Hersteller Artikelnummer: ELA-E-EL-M0166-48
• Green Labware: Nein
• Verpackungseinheit: 48 T
• Mengeneinheit: PAK
• Methode: ELISA