Abbreviation: β-CTx
Target Synonym: b-CTx;bCTXI;bCTX-I;B-Cr;BCL;Type I Collagen C-Telopeptide-Related Fraction;
Target Species: Mouse
Usage: This ELISA kit applies to the in vitro quantitative determination of Mouse β-CTx concentrations in serum, plasma and other biological fluids.
Tested Sample Types: serum, plasma and other biological fluids
Assay Time: 2.5h
Sensitivity: 46.88 pg/mL
Detection Range: 78.13-5000 pg/mL
UNIProt ID: P11087(COL1A1)
Test Principle: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Mouse β-CTx. During the reaction, Mouse β-CTx in the sample or standard competes with a fixed amount of Mouse β-CTx on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Mouse β-CTx. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Mouse β-CTx in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Cross reactivity: This kit recognizes Mouse β-CTx in samples.No significant cross-reactivity or interference between Mouse β-CTx and analogues was observed