English
Mouse CRH(Corticotropin Releasing Hormone) ELISA Kit
Mouse CRH(Corticotropin Releasing Hormone) ELISA Kit
Artikelnummer
ELSE-EL-M0351-48
Verpackungseinheit
48 T
Hersteller
Elabscience Biotechnology
Verfügbarkeit:
wird geladen...
Preis wird geladen...
Abbreviation: CRH
Target Synonym: CRF;Corticoliberin;
Target Species: Mouse
Usage: This ELISA kit applies to the in vitro quantitative determination of Mouse CRH concentrations in serum, plasma and other biological fluids.
Tested Sample Types: serum, plasma and other biological fluids
Assay Time: 2.5h
Sensitivity: 0.19 ng/mL
Detection Range: 0.31-20 ng/mL
UNIProt ID: Q8CIT0
Test Principle: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Mouse CRH. During the reaction, Mouse CRH in the sample or standard competes with a fixed amount of Mouse CRH on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Mouse CRH. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Mouse CRH in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Cross reactivity: This kit recognizes Mouse CRH in samples.No significant cross-reactivity or interference between Mouse CRH and analogues was observed
Target Synonym: CRF;Corticoliberin;
Target Species: Mouse
Usage: This ELISA kit applies to the in vitro quantitative determination of Mouse CRH concentrations in serum, plasma and other biological fluids.
Tested Sample Types: serum, plasma and other biological fluids
Assay Time: 2.5h
Sensitivity: 0.19 ng/mL
Detection Range: 0.31-20 ng/mL
UNIProt ID: Q8CIT0
Test Principle: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Mouse CRH. During the reaction, Mouse CRH in the sample or standard competes with a fixed amount of Mouse CRH on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Mouse CRH. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Mouse CRH in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Cross reactivity: This kit recognizes Mouse CRH in samples.No significant cross-reactivity or interference between Mouse CRH and analogues was observed
Verwandte Artikel
Markieren Sie die Artikel, um Sie dem Warenkorb hinzuzufügen oder
Immer für Sie da
Gernot Ensinger, M.Sc.
Laurent Haze
