Mouse FOXO1 (Forkhead Box Protein O1) CLIA Kit

Abbreviation: FOXO1

Target Synonym: FOX-O1;FKH1;FKHR;FOXO1A;

Target Species: Mouse

Usage: This ELISA kit applies to the in vitro quantitative determination of Mouse FOXO1 concentrations in Tissue homogenates,cell lysates and other biological fluids.

Tested Sample Types: Tissue homogenates,cell lysates and other biological fluids

Assay Time: 3.5h

Sensitivity: 18.75 pg/mL

Detection Range: 31.25-2000 pg/mL

UNIProt ID: Q9R1E0

Test Principle: This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse FOXO1. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse FOXO1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse FOXO1, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Mouse FOXO1. You can calculate the concentration of Mouse FOXO1 in the samples by comparing the RLU value of the samples to the standard curve.

Cross reactivity: This kit recognizes Mouse FOXO1 in samples.No significant cross-reactivity or interference between Mouse FOXO1 and analogues was observed

• Artikelnummer: ELSE-CL-M0449-48
• Hersteller: Elabscience Biotechnology
• Hersteller Artikelnummer: ELA-E-CL-M0449-48
• Green Labware: Nein
• Verpackungseinheit: 48 T
• Mengeneinheit: PAK
• Methode: Chemiluminescence Immunoassays (CLIA)