Mouse GHRL(Ghrelin) ELISA Kit

Abbreviation: GHRL

Target Synonym: GHRL;MTLRP;Ghrelin;

Target Species: Mouse

Usage: This ELISA kit applies to the in vitro quantitative determination of Mouse GHRL concentrations in serum, plasma and other biological fluids.

Tested Sample Types: serum, plasma and other biological fluids

Assay Time: 2.5h

Sensitivity: 0.1 ng/mL

Detection Range: 0.16-10 ng/mL

UNIProt ID: Q9EQX0

Test Principle: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Mouse GHRL. During the reaction, Mouse GHRL in the sample or standard competes with a fixed amount of Mouse GHRL on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Mouse GHRL. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Mouse GHRL in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Cross reactivity: This kit recognizes Mouse GHRL in samples.No significant cross-reactivity or interference between Mouse GHRL and analogues was observed

• Artikelnummer: ELSE-EL-M0551-48
• Hersteller: Elabscience Biotechnology
• Hersteller Artikelnummer: ELA-E-EL-M0551-48
• Green Labware: Nein
• Verpackungseinheit: 48 T
• Mengeneinheit: PAK
• Methode: ELISA