Mouse IgG1(Immunoglobulin G1) ELISA Kit

Mouse IgG1(Immunoglobulin G1) ELISA Kit
Artikelnummer
ELSE-EL-M3035-48
Verpackungseinheit
48 T
Hersteller
Elabscience Biotechnology

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Abbreviation: IgG1

Target Species: Mouse

Usage: This ELISA kit applies to the in vitro quantitative determination of Mouse IgG1 concentrations in serum, plasma and other biological fluids.

Tested Sample Types: serum, plasma and other biological fluids

Assay Time: 3.5h

Sensitivity: 3.75 ng/mL

Detection Range: 6.25-400 ng/mL

UNIProt ID: P01868

Test Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IgG1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IgG1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IgG1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IgG1. You can calculate the concentration of Mouse IgG1 in the samples by comparing the OD of the samples to the standard curve.

Cross reactivity: This kit recognizes Mouse IgG1 in samples.No significant cross-reactivity or interference between Mouse IgG1 and analogues was observed
Mehr Informationen
Artikelnummer ELSE-EL-M3035-48
Hersteller Elabscience Biotechnology
Hersteller Artikelnummer ELA-E-EL-M3035-48
Green Labware Nein
Verpackungseinheit 48 T
Mengeneinheit PAK
Methode ELISA
Produktinformation (PDF) Download
MSDS (PDF) Download