Mouse PF4(Platelet Factor 4) ELISA Kit

Abbreviation: PF4

Target Synonym: CXCL4

Target Species: Mouse

Usage: This ELISA kit applies to the in vitro quantitative determination of Mouse PF4 concentrations in serum, plasma and other biological fluids.

Tested Sample Types: serum, plasma and other biological fluids

Assay Time: 3.5h

Sensitivity: 18.75 pg/mL

Detection Range: 31.25-2000 pg/mL

UNIProt ID: Q9Z126

Test Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse PF4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse PF4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse PF4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse PF4. You can calculate the concentration of Mouse PF4 in the samples by comparing the OD of the samples to the standard curve.

Cross reactivity: This kit recognizes Mouse PF4 in samples.No significant cross-reactivity or interference between Mouse PF4 and analogues was observed

• Artikelnummer: ELSE-EL-M3080-96
• Hersteller: Elabscience Biotechnology
• Hersteller Artikelnummer: ELA-E-EL-M3080-96
• Green Labware: Nein
• Verpackungseinheit: 96 T
• Mengeneinheit: STK
• Methode: ELISA