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Porcine sC5b-9(Soluble Terminal Complement Complex C5b-9) ELISA Kit
Porcine sC5b-9(Soluble Terminal Complement Complex C5b-9) ELISA Kit
Artikelnummer
ELSE-EL-P0485-48
Verpackungseinheit
48 T
Hersteller
Elabscience Biotechnology
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Abbreviation: sC5b-9
Target Species: Porcine
Usage: This ELISA kit applies to the in vitro quantitative determination of Porcine sC5b-9 concentrations
Tested Sample Types: Serum, plasma and other biological fluids
Assay Time: 3.5h
Sensitivity: 4.69 ng/mL
Detection Range: 7.81-500 ng/mL
Test Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Porcine sC5b-9. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Porcine sC5b-9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Porcine sC5b-9, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Porcine sC5b-9. You can calculate the concentration of Porcine sC5b-9 in the samples by comparing the OD of the samples to the standard curve.
Cross reactivity: This kit recognizes Porcine sC5b-9 in samples. No significant cross-reactivity or interference between Porcine sC5b-9 and analogues was observed
Target Species: Porcine
Usage: This ELISA kit applies to the in vitro quantitative determination of Porcine sC5b-9 concentrations
Tested Sample Types: Serum, plasma and other biological fluids
Assay Time: 3.5h
Sensitivity: 4.69 ng/mL
Detection Range: 7.81-500 ng/mL
Test Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Porcine sC5b-9. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Porcine sC5b-9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Porcine sC5b-9, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Porcine sC5b-9. You can calculate the concentration of Porcine sC5b-9 in the samples by comparing the OD of the samples to the standard curve.
Cross reactivity: This kit recognizes Porcine sC5b-9 in samples. No significant cross-reactivity or interference between Porcine sC5b-9 and analogues was observed
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