QuicKey Human IgE(Immunoglobulin E) ELISA Kit

Abbreviation: IgE

Target Species: Human

Usage: This ELISA kit applies to the in vitro quantitative determination of Human IgE concentrations in serum, plasma.

Tested Sample Types: serum, plasma

Assay Time: 2.5h

Sensitivity: 0.27 ng/mL

Detection Range: 0.78-50 ng/mL

Test Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IgE. Samples (or Standards) are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IgE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IgE, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of Human IgE. You can calculate the concentration of Human IgE in the samples by comparing the OD of the samples to the standard curve.

Cross reactivity: This kit recognizes Human IgE in samples.No significant cross-reactivity or interference between Human IgE and analogues was observed

• Artikelnummer: ELSE-TSEL-H0016-48
• Hersteller: Elabscience Biotechnology
• Hersteller Artikelnummer: ELA-E-TSEL-H0016-48
• Verpackungseinheit: 48 T
• Mengeneinheit: PAK
• Methode: ELISA