Rat Ang-II(Angiotensin II) ELISA Kit

Abbreviation: Ang-Ⅱ

Target Synonym: Ang-II;Angiotensin-2;

Target Species: Rat

Usage: This ELISA kit applies to the in vitro quantitative determination of Rat Ang-Ⅱ concentrations in serum, plasma and other biological fluids.

Tested Sample Types: serum, plasma and other biological fluids

Assay Time: 2.5h

Sensitivity: 9.38 pg/mL

Detection Range: 15.63-1000 pg/mL

UNIProt ID: P01015(Angiotensinogen,25 – 32)

Test Principle: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat Ang-Ⅱ. During the reaction, Rat Ang-Ⅱ in the sample or standard competes with a fixed amount of Rat Ang-Ⅱ on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat Ang-Ⅱ. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat Ang-Ⅱ in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Cross reactivity: This kit recognizes Rat Ang-Ⅱ in samples.No significant cross-reactivity or interference between Rat Ang-Ⅱ and analogues was observed

• Artikelnummer: ELSE-EL-R1430-48
• Hersteller: Elabscience Biotechnology
• Hersteller Artikelnummer: ELA-E-EL-R1430-48
• Green Labware: Nein
• Verpackungseinheit: 48 T
• Mengeneinheit: PAK
• Methode: ELISA