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Rat COL2(Collagen Type II) ELISA Kit
Rat COL2(Collagen Type II) ELISA Kit
Artikelnummer
ELSE-EL-R0234-48
Verpackungseinheit
48 T
Hersteller
Elabscience Biotechnology
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Abbreviation: COL2
Target Synonym: COL2
Target Species: Rat
Usage: This ELISA kit applies to the in vitro quantitative determination of Rat COL2 concentrations in serum, plasma and other biological fluids.
Tested Sample Types: serum, plasma and other biological fluids
Assay Time: 3.5h
Sensitivity: 37.5 pg/mL
Detection Range: 62.5-4000 pg/mL
UNIProt ID: P05539
Test Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat COL2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat COL2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat COL2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat COL2. You can calculate the concentration of Rat COL2 in the samples by comparing the OD of the samples to the standard curve.
Cross reactivity: This kit recognizes Rat COL2 in samples. No significant cross-reactivity or interference between Rat COL2 and analogues was observed
Target Synonym: COL2
Target Species: Rat
Usage: This ELISA kit applies to the in vitro quantitative determination of Rat COL2 concentrations in serum, plasma and other biological fluids.
Tested Sample Types: serum, plasma and other biological fluids
Assay Time: 3.5h
Sensitivity: 37.5 pg/mL
Detection Range: 62.5-4000 pg/mL
UNIProt ID: P05539
Test Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat COL2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat COL2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat COL2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat COL2. You can calculate the concentration of Rat COL2 in the samples by comparing the OD of the samples to the standard curve.
Cross reactivity: This kit recognizes Rat COL2 in samples. No significant cross-reactivity or interference between Rat COL2 and analogues was observed
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Gernot Ensinger, M.Sc.
Laurent Haze
