Rat DHEA-S(Dehydroepiandrosterone Sulfate) ELISA Kit

Abbreviation: DHEA-S

Target Species: Rat

Usage: This ELISA kit applies to the in vitro quantitative determination of Rat DHEA-S concentrations in serum, plasma and other biological fluids.

Tested Sample Types: serum, plasma and other biological fluids

Assay Time: 2.5h

Sensitivity: 18.75 pg/mL

Detection Range: 31.25-2000 pg/mL

Test Principle: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat DHEA-S. During the reaction, Rat DHEA-S in the sample or standard competes with a fixed amount of Rat DHEA-S on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat DHEA-S. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat DHEA-S in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Cross reactivity: This kit recognizes Rat DHEA-S in samples.No significant cross-reactivity or interference between Rat DHEA-S and analogues was observed

• Artikelnummer: ELSE-EL-R0325-48
• Hersteller: Elabscience Biotechnology
• Hersteller Artikelnummer: ELA-E-EL-R0325-48
• Verpackungseinheit: 48 T
• Mengeneinheit: PAK
• Methode: ELISA