Tested Sample Types: serum, plasma, urine,animal tissue and cell samples
Assay Time: 25 min
Sensitivity: 9.22 U/L
Detection Range: 9.22-100 U/L
Test Principle: The chromogenic agent can be catalyzed by acid phosphatase to produce p-nitrophenol in acidic condition. P-nitrophenol has a maximum absorption at 405 nm. The activity of TRAP can be calculated by measuring the amount of produced p-nitrophenol. The activity of acid phosphatase being detected in the presence of tartric acid is regarded as TRAP activity.