ULK1 Kinase Assay Kit

Products from BPS Bioscience require a minimum order value above 400€

Application: Study enzyme kinetics and screen small molecular inhibitors for drug discovery and High Throughput (HTS) applications.

Background: ULK1  (unc-51 like autophagy activating kinase 1) is a serine/threonine protein kinase that plays a critical role during the initial stages of autophagy in response to nutrient starvation. The conserved C-terminal domain of ULK1 controls the  function and the localization of the protein. Knockdown of ULK1 inhibits the autophagic response  and prevents rapamycin-induced autophagy, consistent with a role downstream of mTOR. ULK1 forms a complex with FIP200 and ATG13 and this complex is essential for starvation-induced autophagy (1). Both FIP200 and ATG13 are critical for correct localization of ULK1 to the pre-autophagosome and stability of the ULK1 protein (2). ULK1 is phosphorylated upon activation of the mTOR pathway in a nutrient starvation-regulated manner. Alterations in ULK signaling pathways may be involved in the formation of autophagy-regulated Lewi bodies, which have been associated with Parkinson's disease (3).

Contraindications: The final concentration of DMSO in the assay should not exceed 1%.

Description: The ULK1 Kinase Assay Kit is designed to measure ULK1 activity for screening and profiling applications, using Kinase-Glo® MAX as a detection reagent.

Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.

Supplied As: The ULK1 Assay Kit comes in a convenient 96-well format, with enough purified ULK1, MBP substrate, ATP, and kinase assay buffer for 96 enzyme reactions.

Uniprot: O75385

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

• Artikelnummer: BPS78362
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 78362
• Verpackungseinheit: 96 rxns.
• Mengeneinheit: PAK