Tested Sample Types: serum, animal tissue and urine samples
Assay Time: 45 min
Sensitivity: 0.032 ug/mL
Detection Range: 0.032-10 ug/mL
Test Principle: The sample is hydrolyzed to generate free HYP, and hydroxyproline can produce oxidation product under the action of oxidizing agent. The generated oxidation product can react with chromogenic agent to produce burgundy. The concentration of hydroxyproline can be calculated by measuring the OD value at 558 nm.