Human CCL22 - MDC ELISA Kit

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of the 2000pg/ml,1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml human MDC standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human MDC standard solution and each sample be measured in duplicate.

Sensitivity: <1 pg/ml

Detection Range: 31.2 pg/ml - 2000 pg/ml

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Immunogen: Expression system for standard: E.coli; Immunogen sequence:  G25-Q93

Purity and Specificity: Natural and recombinant human MDC. There is no detectable cross-reactivity with other relevant proteins.

Background: Macrophage-derived chemokine(MDC), also called Chemokine, cc motif, ligand 22(CCL22) or Small inducible cytokine subfamily A, member 22(SCY22). MDC is a recently identified member of the CC chemokine family. It is not closely related to other chemokines, sharing most similarity with thymus- and activation-regulated chemokine(TARC), which contains 37% identical amino acids. In addition, MDC gene is mapped to chromosome 16q13, the same position reported for the TARC gene. MDC has the four-cysteine motif and other highly conserved residues characteristic of CC chemokines, but it shares<35% identity with any of the known chemokines. Recombinant MDC was expressed in Chinese hamster ovary cells and purified by heparin-Sepharose chromatography. MDC is highly expressed in macrophages and in monocyte-derived dendritic cells, but not in monocytes, natural killer cells, or several cell lines of epithelial, endothelial, or fibroblast origin. High expression was also detected in normal thymus and less expression in lung and spleen. MDC is thus a unique member of the CC chemokine family that may play a fundamental role in the function of dendritic cells, natural killer cells, and monocytes.

Low Endotoxin: No

• Artikelnummer: ROCKOA0255
• Hersteller: Rockland
• Hersteller Artikelnummer: KOA0255
• Verpackungseinheit: 1 Kit
• Mengeneinheit: PAK
• Reaktivität: Human
• Methode: ELISA
• Human Gene ID: 6367
• Konjugat: Unconjugated