Human HGF ELISA Kit

Human HGF ELISA Kit
Artikelnummer
ROCKOA0186
Verpackungseinheit
1 Kit
Hersteller
Rockland

Verfügbarkeit: wird geladen...
Preis wird geladen...
Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of the 8000pg/ml,4000pg/ml, 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml human HGF standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma(EDTA, citrate) to each empty well. It is recommended that each human HGF standard solution and each sample be measured in duplicate.

Sensitivity: <5 pg/ml

Detection Range: 125 pg/ml - 8000 pg/ml

General Disclaimer Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen: Expression system for standard: sf21; Immunogen sequence: Q32-R494(alpha)&V495-S728(beta)

Purity and Specificity: Natural and recombinant human HGF. There is no detectable cross-reactivity with other relevant proteins.

Background: Hepatocyte growth factor(HGF) is the most potent mitogen for mature parenchymal hepatocytes in primary culture, and seems to be a hepatotrophic factor that acts as a trigger for liver regeneration after partial hepatectomy and liver injury. HGF has a relative molecular mass(Mr) of 82,000 and is a heterodimer composed of a large alpha-subunit of Mr 69,000 and a small beta-subunit of Mr 34,000. The protein consists of 728 amino acid residues, including a possible signal peptide at the N-terminus. HGF may serve as a paracrine mediator to control placental development and growth. This growth factor may play an important role as a paracrine mediator of the proliferation of melanocytes and endothelial cells, as well as cells of epithelial origin. The gene encoding the human HGF is assigned to human chromosome 7.

Low Endotoxin: No
Mehr Informationen
Artikelnummer ROCKOA0186
Hersteller Rockland
Hersteller Artikelnummer KOA0186
Verpackungseinheit 1 Kit
Mengeneinheit PAK
Reaktivität Human
Methode ELISA
Human Gene ID 3082
Konjugat Unconjugated
Produktinformation (PDF) Download
MSDS (PDF)
×