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Human IgE Chemiluminescent ELISA Kit
Human IgE Chemiluminescent ELISA Kit
Artikelnummer
BPS82720
Verpackungseinheit
96 reactions
Hersteller
BPS Bioscience
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Products from BPS Bioscience require a minimum order value above 400€
Application: Quantify human IgE in cell culture supernatants.
Background: IgE (immunoglobulin E) is an immunoglobulin isotype that is found only in mammals. It can be found at low levels in the blood of normal individuals and plays a role in immune responses to parasites, toxins, cancer and hypersensitivity reactions to allergens. It is linked to allergic asthma, sinusitis, allergic rhinitis, atopic dermatitis (AD), and can trigger anaphylaxis. IgE can bind to the high affinity IgE receptor (FcγRI ) or the low affinity FcγRII (also known as CD23). In the presence of an allergen, IgE binds to FcγRI present in mast cells and basophils, triggering the release of histamine, leukotrienes and interleukins. CD23 has a broader expression pattern, including macrophages and B cells, and plays a role in the regulation of IgE synthesis, serum clearance and antigen presentation. IgE is highly glycosylated, and the glycosylation of N394 in human IgE is crucial for receptor binding. IgE is found at high levels in patients with SLE (systemic lupus erythematosus), RA (reumathoid arthritis) and psoriasis. The development of therapeutic antibodies targeting IgE, such as omalizumab, may be useful for the treatment of IgE- mediated inflammation and allergic diseases. In addition, the properties of IgE can be used to increase ADCC (antibody-dependent cell mediated cytotoxicity) by monocytes and other immune cells, ADCP (antibody dependent cell-mediated phagocytosis) and other mechanisms that target cancer cells.
Contraindications: The Human IgE Chemiluminescent ELISA Kit is compatible with up to 1% final DMSO concentration.
Description: The Human IgE Chemiluminescent ELISA Kit is designed to detect and quantify protein levels of human IgE (immunoglobulin E) captured by pre-matched antibody pairs. This assay kit comes in a convenient 96-well format, with a pre-coated anti-IgE-specific antibody plate, enough recombinant purified IgE standard, and detection reagents for 100 wells.
Figure 1: Human IgE Chemiluminescent ELISA Kit assay principle.
Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
Warnings: Avoid freeze/thaw cycles
Biosafety Level: Not applicable (BSL-1)
References: Vogel M. and Engeroff P., 2024 Antibodies (Basel) 13(3):58.Sutton B., et al., 2019 Antibodies (Basel) 8(1):19.
Application: Quantify human IgE in cell culture supernatants.
Background: IgE (immunoglobulin E) is an immunoglobulin isotype that is found only in mammals. It can be found at low levels in the blood of normal individuals and plays a role in immune responses to parasites, toxins, cancer and hypersensitivity reactions to allergens. It is linked to allergic asthma, sinusitis, allergic rhinitis, atopic dermatitis (AD), and can trigger anaphylaxis. IgE can bind to the high affinity IgE receptor (FcγRI ) or the low affinity FcγRII (also known as CD23). In the presence of an allergen, IgE binds to FcγRI present in mast cells and basophils, triggering the release of histamine, leukotrienes and interleukins. CD23 has a broader expression pattern, including macrophages and B cells, and plays a role in the regulation of IgE synthesis, serum clearance and antigen presentation. IgE is highly glycosylated, and the glycosylation of N394 in human IgE is crucial for receptor binding. IgE is found at high levels in patients with SLE (systemic lupus erythematosus), RA (reumathoid arthritis) and psoriasis. The development of therapeutic antibodies targeting IgE, such as omalizumab, may be useful for the treatment of IgE- mediated inflammation and allergic diseases. In addition, the properties of IgE can be used to increase ADCC (antibody-dependent cell mediated cytotoxicity) by monocytes and other immune cells, ADCP (antibody dependent cell-mediated phagocytosis) and other mechanisms that target cancer cells.
Contraindications: The Human IgE Chemiluminescent ELISA Kit is compatible with up to 1% final DMSO concentration.
Description: The Human IgE Chemiluminescent ELISA Kit is designed to detect and quantify protein levels of human IgE (immunoglobulin E) captured by pre-matched antibody pairs. This assay kit comes in a convenient 96-well format, with a pre-coated anti-IgE-specific antibody plate, enough recombinant purified IgE standard, and detection reagents for 100 wells.
Figure 1: Human IgE Chemiluminescent ELISA Kit assay principle.Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
Warnings: Avoid freeze/thaw cycles
Biosafety Level: Not applicable (BSL-1)
References: Vogel M. and Engeroff P., 2024 Antibodies (Basel) 13(3):58.Sutton B., et al., 2019 Antibodies (Basel) 8(1):19.
| Artikelnummer | BPS82720 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 82720 |
| Verpackungseinheit | 96 reactions |
| Mengeneinheit | PAK |
| Produktinformation (PDF) | Download |
| MSDS (PDF) |
|
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