LIVE-Step™ Cell Assay System

Products from BPS Bioscience require a minimum order value above 400€

Application: Determine metabolically active, viable cells values in mammalian culture by using a standard curve.

Background: Determining viable cell numbers in cell culture is an essential measure across various biological fields. Traditionally, manual counting with trypan blue was the standard. While automated cell counters offer convenience, they might not be ideal for high-throughput applications. Enzyme-based methods, such as dehydrogenase assays, can provide a more scalable solution. By measuring enzymatic activity, researchers can indirectly assess cell viability. ATP-based luminescence measurements offer a direct and sensitive approach. Using thermostable firefly luciferase, this method provides a stable readout for extended periods. Compared to other techniques, ATP-based luminescence is generally more sensitive, reliable, and easier to implement, making it well-suited for high-throughput screening.

Contraindications: The presence of organic solvents or other compounds in the culture can result in luciferase inhibition and impact the readings.If organic solvents or other compounds are used, it is recommended that an initial test is performed to determine the impact of the compound alone. For example, prepare a reaction containing LIVE-Step™ Cell Assay Solution, 1 µM ATP and the Diluent Reagent used to prepare the compound, and a reaction containing LIVE-Step™ Cell Assay Solution, 1 µM ATP and the compound of interest in Diluent Reagent at the maximum concentration to be tested. If a decrease in signal is observed, it may be necessary to perform a titration of the compound to assess the value at which no impact is seen, or to use a control for each concentration being tested.It is recommended that the edge wells of the plate are not used, to avoid temperature gradients that can affect the signal.ATP contamination should be avoided, by using aseptic technique while performing the assay and avoiding addition of extracellular ATP in the culture media.Avoid exposure of the reagent to light and excessive heat during storage.

Description: The LIVE-Step™ Cell Assay System is designed for high-throughput, homogeneous, sensitive luminescence quantification of metabolically active, viable mammalian cells. It consists of a single ready-to-use solution to lyse the cells and measure the amount of ATP present in culture in one step. Detection is linear up to >100,000 suspension cells and >50,000 adherent cells with an R² value greater than 0.99. The signal is stable for longer than 5 hours (T_1/2 >300 min) allowing for experimental flexibility. This product is compatible with multiple media formulations and the presence of phenol red, and stable after multiple freeze-thaw cycles.

Storage Stability: Reagents will perform optimally for up to 6 months from date of receipt when the materials are stored as directed. Upon first thaw, store in aliquots at -20°C. The reagent may be subjected to several freeze/thaw cycles with no effect on functionality, but it is recommended that freeze/thaw cycles be avoided whenever possible. 

Warnings: Protect from light. Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

• Artikelnummer: BPS82648-3
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 82648-3
• Verpackungseinheit: 10 x 10 ml
• Mengeneinheit: PAK