Mouse CCL3 - MIP1 alpha ELISA Kit

Mouse CCL3 - MIP1 alpha ELISA Kit
Artikelnummer
ROCKOA0258
Verpackungseinheit
1 Kit
Hersteller
Rockland

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Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of the 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.3pg/ml, 15.6pg/ml, 7.8pg/ml mouse CCL3 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. We recommend that each mouse CCL3 standard solution and each sample is measured in duplicate.

Concentration Value: 1 Kit

Sensitivity: <10 pg/ml

Detection Range: 7.8 pg/ml - 500 pg/ml

General Disclaimer Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen: Expression system for standard: E.coli; Immunogen sequence:  A24-A92

Purity and Specificity: Natural and recombinant mouse CCL3. There is no detectable cross-reactivity with other relevant proteins.

Background: Chemokine(C-C motif) ligand 3(CCL3), so known as Macrophage inflammatory protein-1alpha(MIP-1 alpha), is a protein that in humans is encoded by the CCL3 gene. It is a cytokine which belongs to the CC chemokine family that is involved in the acute inflammatory state in the recruitment and activation of polymorphonuclear leukocytes. This gene is mapped to 17q12. CCL3 plays a role in inflammatory responses through binding to the receptors CCR1, CCR4 and CCR5. It has been found that the activation of CCR5 by CCL3 directly and independently activates a G-protein signaling pathway through GNAI2 and a tyrosine phosphorylation signaling pathway through JAK2.

Low Endotoxin: No
Mehr Informationen
Artikelnummer ROCKOA0258
Hersteller Rockland
Hersteller Artikelnummer KOA0258
Verpackungseinheit 1 Kit
Mengeneinheit PAK
Reaktivität Mouse (Murine)
Methode ELISA
Human Gene ID 20302
Konjugat Unconjugated
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