Notes: This ERK1/2 (Extreme Phospho-Thr202/Tyr204) TR-FRET Assay Kit uses a homogeneous TR-FRET assay method amenable to rapid measurement of phosphorylated protein levels in cells. The signal is stable at room temperature for at least 24 hours, affording flexibility in read times. The amount of reagents provided is sufficient for testing either 96 or 480 phosphorylated protein wells, depending on the size of the kit. THUNDER™ TR-FRET Cell Signaling Assay Kits are based on Bioauxilium’s enhanced proprietary time-resolved Förster resonance energy transfer (TR-FRET) technology.