CSL (CBF1/RBP-Jk) Luciferase Reporter Lentivirus (Notch Signaling Pathway)

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Applications: Screen for activators or inhibitors of the Notch signaling pathway in cells using luciferase activity as readout.Generate CSL-responsive Luciferase Reporter cell pools or stable cell lines by puromycin selection.Generate a Notch1dE/CSL Luciferase Reporter cell system when used in combination with Notch1dE Lentivirus (BPS Bioscience #78747).

Background: The Notch signaling pathway controls cell fate decisions in vertebrate and invertebrate tissues, and it is involved in embryonic development, tissue homeostasis, and regulation of immune and angiogenic systems. Notch signaling is triggered through the binding of a transmembrane ligand, present in opposing cells, to one of the four existing Notch transmembrane receptors (Notch1/ Notch2/Notch3/Notch4). This results in proteolytic cleavage of the Notch receptor, releasing the constitutively active intracellular domain of Notch (NICD). NICD translocate to the nucleus and associates with the transcription factor CSL (CBF1/RBPJκ/Suppressor of Hairless/Lag-1) and coactivator Mastermind to turn on the transcription of Notch-responsive genes. Dysfunction of Notch signaling has severe consequences, from developmental disorders to cancer (such as T cell acute lymphoblastic leukemia, T-ALL, and urothelial bladder cancer). The use of Notch inhibitors, mainly gamma-secretase inhibitors, has shown promise in cancer therapy and in regenerating tissues. Further studies will deepen our understanding of Notch signaling and will benefit future therapeutic approaches.

Description: The CSL (CBF1/RBP-Jk) Luciferase Reporter Lentivirus (Notch Signaling Pathway) are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce most mammalian cells, including primary and non-dividing cells. These viruses contain a firefly luciferase reporter driven by multiple copies of the CSL responsive element (CBF1/RBPJκ/Suppressor of Hairless/Lag-1) located upstream of the minimal TATA promoter. The lentiviruses also contain a puromycin selection marker (Figure 1). After transduction, Notch signaling can be monitored by measuring luciferase activity.

Formulation: The lentivirus particles were produced in HEK293T cells. They are supplied in cell culture medium containing 90% DMEM + 10% FBS. Virus particles can be packaged in custom formulations by special request, for an additional fee.

Storage Stability: Lentiviruses are shipped with dry ice. For long-term storage, it is recommended to store the lentiviruses at -80°C. Avoid repeated freeze-thaw cycles. Titers can drop significantly with each freeze-thaw cycle.

Supplied As: Two vials (500 µl x 2) of lentivirus at a titer ≥10⁷ TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.

Warnings: Avoid freeze/thaw cycles

Biosafety Level: BSL-2

References: Lu F.M., et al., 1996 Natl. Acad. Sci. USA 93(11): 5663-5667.Kanungo J., et al., 2008 Neurochem. 106: 2236-48.Cao L. et al., 2023 Blood Adv. 1182/bloodadvances.2023010380

• Artikelnummer: BPS78746
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 78746
• Verpackungseinheit: 500 µl x 2
• Mengeneinheit: STK