Description: EvaRuby™ is the first visible red fluorogenic DNA dye for qPCR and HRM, with Ex/Em at 480/610 nm upon binding to dsDNA. It can be combined with fluorescent probes for probe-based qPCR with high resolution melt analysis in the same reaction. 20X in water, 2x1 mL (2000 x 20 uL reactions).
Product Origin: Synthetic
Product features: Visible red qPCR dye for qPCR, HRM*, and LAMP; Unique spectral properties: Ex/Em 480/613 nm (with DNA) with minimal cross-talk in neighboring detection channels; Combine probe-based qPCR with HRM* analysis in the same reaction; Compatible with ROX* and VeriFluor™ Far-Red passive reference dyes; Requires detection with a custom* filter combination of green ~488 nm excitation and orange ~610 nm emission filters [*Compatibility may vary by instrument]