NFĸB Luciferase-eGFP Reporter Lentivirus

Products from BPS Bioscience require a minimum order value above 400€

Application: Screen for activators or inhibitors of NFκB signaling pathway in transduced target cells.Generate NFκB luciferase-eGFP reporter cell pools or stable cell lines following puromycin selection.

Background: The role of NFκB (nuclear factor kappa-light chain enhancer of activated B cells) activation is well-characterized in canonical (classical) and noncanonical (alternative) signaling pathways of inflammation. Two major forms of innate immune sensors are Toll-like receptors (TLR) and NOD/CATERPILLER proteins. Mutations in NOD2 (nucleotide-binding oligomerization domain-containing protein 2) have been linked to chronic autoinflammatory and autoimmune diseases, such as Crohn’s disease and Blaus syndrome. Studying the canonical and noncanonical NF-κB pathways and the influence of TLR pathways and NOD2 mutations can further our understanding of autoimmune regulation. The use of luciferase and eGFP (enhanced green fluorescence protein) reporter allows for easy read outs in cellular assays.

Description: The NFĸB Luciferase-eGFP Reporter Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce almost all types of mammalian cells, including primary and nondividing cells. The particles contain a firefly luciferase and eGFP (enhanced green fluorescent protein) cassette driven by the NFĸB (nuclear factor kappa-light-chain-enhancer of activated B cells) response element located upstream of the minimal TATA promoter. These lentiviruses also transduce a puromycin selection marker. After transduction, activation of the NFĸB signaling pathway in the target cells can be monitored by measuring luciferase activity or/and eGFP expression.

Formulation: The lentiviruses were produced from HEK293T cells. Supplied in medium containing 90% DMEM + 10% FBS. Virus particles can be packaged in custom formulations and produced at higher titers by special request, for an additional fee.

Storage Stability: Lentiviruses are shipped with dry ice. For long-term storage, it is recommended to store the lentiviruses at -80°C for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles. Titers can drop significantly with each freeze/thaw cycle.

Supplied As: Two vials (500 µl x 2) of NFκB Luciferase-eGFP Reporter Lentivirus at ≥107 TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: BSL-2

References: Pessara U., Koch N., 1990 Mol Cell Biol. 10(8):4146-4154.Baeuerle P.A., 1998 Curr Biol. 8(1): R19-R22.Treisman R., 1992 Trends Biochem Sci. 17(10): 423-426.

• Artikelnummer: BPS82336
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 82336
• Verpackungseinheit: 500 µl x 2
• Mengeneinheit: PAK