STAT3 Luciferase Reporter Lentivirus

Products from BPS Bioscience require a minimum order value above 400€

Applications: Screen for activators or inhibitors of STAT3 signaling pathway in the transduced target cellsGeneration of STAT3 luciferase reporter stable cell line

Description: The STAT3 Luciferase Reporter Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene under the control of STAT3-responsive element located upstream of the minimal TATA promoter. After transduction, activation of the STAT3 signaling pathway in the target cells can be monitored by measuring the luciferase activity.

Formulation: The lentiviruses were produced from HEK293T cells and are provided in medium containing 90% DMEM + 10% FBS.

Storage Stability: Lentiviruses are shipped with dry ice. For long term storage, it is recommended to store the virus at -80°C. Avoid repeated freeze-thaw cycles. Titers can drop significantly with each freeze-thaw cycle.

Supplied As: Two vials (500 µl x 2) of STAT3 luciferase reporter lentivirus at a titer ≥5 x 10^6 TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: BSL-2

References: 1. Pessara, U., Koch, N. (1990) Tumor necrosis factor alpha regulates expression of the major histocompatibility complex class II-associated invariant chain by binding of an NF-κB-like factor to a promoter element. Mol Cell Biol. 10(8):4146-4154.2. Baeuerle, P.A. (1998) Pro-inflammatory signaling: last pieces in the NF-κB puzzle? Curr Biol. 8(1):R19-R22.

• Artikelnummer: BPS79744
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 79744
• Verpackungseinheit: 500 µl x 2
• Mengeneinheit: STK