LAIR1 Lentivirus

Products from BPS Bioscience require a minimum order value above 400€

Applications: Expression of human LAIR1 in cells of interest.Generate cell pools or stable cell lines expressing human LAIR1 following puromycin selection.

Background: LAIR1, also known as leukocyte-associated immunoglobulin-like receptor 1 or CD305 (cluster of differentiation 305) is an inhibitory receptor present in NK (natural killer) cells, T and B cells. In NK cells it binds to SHP-1 (Src homology 2 domain-containing protein tyrosine phosphatase 1) and SHP-2 phosphatases, resulting in inhibition of NK-mediated cytotoxicity. LAIR1 contributes to CD8⁺ T- cell exhaustion in cancer, by binding to collagen and suppressing T cell activity via SHP-1. The increased expression of collagen and LAIR1 in lung cancer patients is linked to a poor prognostic. In addition, patients that develop resistance to PD-1 (programmed death 1) inhibitor treatment present LAIR1 expression. The use of inhibitors that prevent the binding of LAIR1 to its ligand has been shown to increase anti-tumor immunity. Further studies are needed to fully elucidate the role of LAIR1 in cancer and develop efficacious therapies targeting LAIR1 alone or in combination with PD-1 inhibitors.

Description: LAIR1 (leukocyte-associated immunoglobulin-like receptor 1) Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles ready to transduce nearly all types of mammalian cells, including primary and non-dividing cells. These particles contain human LAIR1 (NM_ 002287.6) driven by a CMV promoter and puromycin selection marker.

Formulation: The lentivirus particles were produced in HEK293T cells in medium containing 90% DMEM + 10% FBS. Virus particles can be packaged in custom formulations by special request, for an additional fee.  

Storage Stability: Lentiviruses are shipped with dry ice. For long-term storage, it is recommended to store the lentiviruses at -80°C. Avoid repeated freeze-thaw cycles. Titers can drop significantly with each freeze-thaw cycle.

Supplied As: Two vials (500 µl x 2) of lentivirus at a titer ≥10⁷ TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.

Warnings: Avoid freeze/thaw cycles

Biosafety Level: BSL-2

References: Meyaard L., et al., 1997 Immunity 7(2): 283-90.Aung T., et al., 2023 Cancer Res Commun. 3(3): 471-482.

• Artikelnummer: BPS78903
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 78903
• Verpackungseinheit: 500 µl x 2
• Mengeneinheit: STK