MART-1-Specific TCR Lentivirus (Clone DMF4)

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Application: Use as a positive control for MART-1 TCR evaluation and optimize experimental conditions.Generate MART-1 TCR stable cell line (puromycin resistant).

Background: MART-1 is a differentiation antigen expressed at the surface of melanocytes. A peptide fragment of the protein is found bound to MHC complexes that are recognized by cytotoxic T cells. MART-1 is found in most skin cancers, including melanomas, and is used as a biomarker for diagnostic purposes. Since the expression of the protein is restricted to melanocyte containing tissues (skin and retina), and is not found in other tissues, MART-1 is an attractive target for cancer vaccines and adoptive cell therapy. MART-1 peptide 26-35 is a fragment commonly associated with MHC and recognized by T cell receptors.

Description: The human MART-1-specific T Cell Receptor lentiviruses (clone DMF4) are replication incompetent, HIV-based, VSV-G-pseudotyped lentiviral particles that are ready to infect almost all types of mammalian cells, including primary and non-dividing cells. These viruses transduce a human TCR (clone DMF4) that specifically recognizes antigen MART-1 (Melanoma-associated antigen recognized by T cells-1, or Melan-A), and in which the TCR α chain and β chain are linked by P2A. The lentiviruses also transduce a puromycin selection gene. Figure 1. (A) Schematic of the lenti-vector used to generate the MART-1-specific TCR lentivirus and (B) Construct diagram showing expressed components of the MART-1-specific TCR. TRAV and TRAC correspond to the TCR alpha chain variable and constant regions, respectively, whereas TRBV and TRBC correspond to the TCR beta chain variable and constant regions.

Formulation: The lentiviruses were produced in HEK293T cells in medium containing 90% DMEM + 10% FBS.  Virus particles can be packaged in custom formulations by special request, for an additional fee

Storage Stability: Lentiviruses are shipped with dry ice. For long term storage, it is recommended to store the virus at -80°C. Avoid repeated freeze-thaw cycles. Titers can drop significantly with each freeze-thaw cycle.

Supplied As: ≥2x10⁷TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.

Warnings: Avoid freeze/thaw cycles

Biosafety Level: BSL-2

• Artikelnummer: BPS78678-2
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 78678-2
• Verpackungseinheit: 500 µl x 2
• Mengeneinheit: STK