RFP Lentivirus

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Application: Ideal as a positive control for transduction; useful for transduction optimization.Generation of stable cells expressing RFP upon puromycin selection.

Description: TurboRFP is a red (orange) fluorescent protein derived from sea anemone Entacmaea quadricolor. The RFP Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to infect almost all types mammalian cells, including primary and non-dividing cells. These viruses constitutively express TurboRFP under a CMV promoter. RFP expression and transduction efficiency can easily be verified and optimized via fluorescence microscopy or flow cytometry. RFP has an excitation wavelength of 553 nm, an emission wavelength of 574 nm, and an extinction coefficient of 92,000 M^-1cm^-1.

Formulation: The lentiviruses were produced from HEK293T cells. Supplied in medium containing 90% DMEM + 10% FBS.

Storage Stability: Lentiviruses are shipped with dry ice. For long term storage, it is recommended to store the virus at -80°C. Avoid repeated freeze-thaw cycles. Titers can drop significantly with each freeze-thaw cycle.

Supplied As: Two vials (500 µl x 2) of RFP lentivirus at a titer ≥1 x 10⁷ TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.

Warnings: Avoid repeated freeze/thaw cycles. Titers can drop significantly with each freeze/thaw cycle.Biosafety: The lentiviruses are produced with a SIN (self-inactivation) lentivector which ensures self-inactivation of the lentiviral construct after transduction and integration into the genomic DNA of the target cells. None of the HIV genes (gag, pol, rev) will be expressed in the transduced cells, as they are expressed from packaging plasmids lacking the packing signal. Although the pseudotyped lentiviruses are replication-incompetent, they require the use of a Biosafety Level 2 facility. BPS recommends following all local federal, state, and institutional regulations and using all appropriate safety precautions.

Biosafety Level: BSL-2

• Artikelnummer: BPS78347-P
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 78347-P
• Verpackungseinheit: 500 µl x 2
• Mengeneinheit: STK