Application Note: TrueBlot® Biotin magnetic beads are stored in phosphate buffered saline with detergent. TrueBlot® Biotin magnetic beads can be used in immunoassays or molecular diagnostics when avidin or streptavidin is bound to proteins or oligonucleotides. Biotin magnetic beads can also be used in nucleic acid isolation, protein purification, and cell separations. Biotin magnetic particles are incubated with the avidin or streptavidin labeled solution and then separated by magnets. After the unbound particulates are washed from the particles, the bound avidin are eluted from the particles using the elution buffer. The particles are then magnetically separated from the eluted solution, which is removed manually.
Concentration Value: 5.0 mg/mL
Immunoprecipitation Dilution: User Optimized
Western Blot Dilution: User Optimized
General Disclaimer Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Physical State: Suspension of Magnetic Beads
Background: TrueBlot® Magnetic Beads are uniform, non-aggregating, super-paramagnetic beads consisting of a ferric oxide core functionalized with various silane groups. The super-paramagnetic nanoparticles are coupled with a biomolecule, such as Biotin, and are specifically designed, tested and quality controlled for magnetic separation, isolation and purification of avidin and streptavidin-labeled components using manual or automatic platforms. Biotin magnetic beads are stable, pre-blocked beads with high binding capacity that provide rapid and efficient biomolecule purification from complex samples. Bead mean diameter is ~0.5 μm, bead concentration is 5 mg/mL, and binding capacity is ≥ 30 µg streptavidin/mg of beads.
Low Endotoxin: No
Other: User Optimized
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