Application Note: TrueBlot® Protein G magnetic beads has been tested by IP and SDS-PAGE and can be used for immunoprecipitation (IP) and Co-IP experiments as well as for antibody purification. For IP, target specific antibody is incubated with cell lysate. Protein G magnetic beads are then incubated with antigen-antibody complex at room temperature, washed, and then eluted using elution buffer. The samples are then resolved by SDS-PAGE and analyzed by Western blotting. For antibody purification, Protein G magnetic beads are incubated with the antibody solution and then separated by magnets. After the unbound particulates are washed from the beads, the bound antibodies are eluted from the beads using the elution buffer. The beads are then magnetically separated from the eluted solution, which is removed manually.
Concentration Value: 5mg/ml
Immunoprecipitation Dilution: User Optimized
Western Blot Dilution: User Optimized
General Disclaimer Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Physical State: Suspension of Magnetic Beads
Background: TrueBlot® Magnetic Beads are uniform, non-aggregating, super-paramagnetic beads consisting of a ferric oxide core functionalized with various silane groups. The super-paramagnetic nanoparticles are coupled with a biomolecule, such as Protein G, and are specifically designed, tested and quality controlled for isolation and purification of antibodies, and immunoprecipitation methods using manual or automatic platforms. Protein G, attached to magnetic bead surface, can bind to antibodies from many different species, including mouse, human, rabbit, cow, goat and sheep. Immunoprecipitation assays with Protein G magnetic beads result in high capture efficiencies and high yield of target antigen. Protein G magnetic beads are stable, pre-blocked beads that provide highly purified product. Bead mean diameter is ~0.5 μm, bead concentration is 5.0 mg/mL, and binding capacity is ≥ 80 μg rabbit IgG/mg of beads.
Low Endotoxin: No
Other: User Optimized
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