Application Note: TrueBlot® Streptavidin magnetic beads are stored in phosphate buffered saline with detergent. TrueBlot® Streptavidin magnetic beads can be used for immunoprecipitation (IP) of antigens (using biotinylated antibodies) from a wide variety of sources and to Co-immunoprecipitate interaction complexes using biotinylated antibodies. Streptavidin magnetic beads can also be used for protein-protein interaction in pull-down assays using biotinylated "bait" proteins and for isolation of biotin-labeled DNA-protein complexes from cell or tissue extracts. These beads are also applied for capturing single-stranded-biotinylated DNA oligos and for isolation of biotinylated PCR products.
Concentration Value: 5.0 mg/mL
Immunoprecipitation Dilution: User Optimized
Western Blot Dilution: User Optimized
General Disclaimer Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Physical State: Suspension of Magnetic Beads
Background: TrueBlot® Magnetic Beads are uniform, non-aggregating, super-paramagnetic beads consisting of a ferric oxide core functionalized with various silane groups. The super-paramagnetic nanoparticles are coupled with a biomolecule, such as Streptavidin, and are specifically designed, tested and quality controlled for magnetic purification of biotin-labeled components using manual or automatic platforms. Streptavidin magnetic beads are stable, pre-blocked beads with high binding capacity that provide rapid and efficient biomolecule purification from complex samples. Bead mean diameter is ~0.5 μm, bead concentration is 5.0 mg/mL, and binding capacity is ≥ 60 µg biotin/mg of beads.
Low Endotoxin: No
Other: User Optimized
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