TrueBlot® Anti-Mouse IgG Magnetic Beads

Application Note: TrueBlot® goat Anti-mouse IgG magnetic beads can be used for separation and purification of mouse antibodies from serum or mouse antibody-labeled components, as well as for immunoassays, immunoprecipitation, and IP Western blots. Anti-Mouse IgG Magnetic Beads has been tested in SDS-Page, immunoprecipitation, and western blot. For antibody purification, goat Anti-mouse IgG magnetic beads are incubated with the mouse antibody solution and then separated by magnets. After the unbound particulates are washed from the beads, the bound antibodies are eluted from the beads using the elution buffer. The beads are then magnetically separated from the eluted solution, which is removed manually. For IP, target specific antibody is incubated with goat Anti-mouse IgG magnetic beads. The unbound antibody is washed and the sample containing target antigen is added. After unbound particulates are washed from the beads, the purified protein is eluted from the beads using elution buffer. The samples are then resolved by SDS-PAGE and analyzed by Western blotting.

Concentration Value: 5.0 mg/ml

Immunoprecipitation Dilution: User Optimized

Western Blot Dilution: User Optimized

General Disclaimer Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Physical State: Liquid

Background: TrueBlot® Magnetic Beads are uniform, non-aggregating, super-paramagnetic beads consisting of a ferric oxide core functionalized with various silane groups. The super-paramagnetic nanoparticles are coupled with a biomolecule, such as goat Anti-mouse IgG, and are specifically designed, tested and quality controlled for isolation and purification of mouse IgG, and immunoprecipitation methods using manual or automatic platforms. This antibody binds the heavy chain of mouse IgG and is suitable for immunoassays that utilize a mouse IgG primary polyclonal antibody. Cell separation and sorting can be achieved using a mouse IgG antibody to defined cell surface antigens. The beads have a large surface area with high capture efficiencies. The beads are in suspension and will settle upon storage. Prior to use, mix the vial gently (do not vortex) to ensure delivery of proper bead volume. Bead mean diameter is ~0.5 μm, bead concentration is 5 mg/mL.

Low Endotoxin: No

Other: User Optimized

• Artikelnummer: ROC00-1811-20
• Hersteller: Rockland
• Hersteller Artikelnummer: 00-1811-20
• Verpackungseinheit: 2.0 mL
• Mengeneinheit: STK
• Reaktivität: Mouse (Murine)
• Klonalität: Polyclonal
• Methode: Immunoprecipitation, Western Blotting, SDS-PAGE
• Isotyp: IgG
• Wirt: Goat
• Konjugat: Conjugated