FTO, His-Tag (Sf9 derived) Recombinant

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Encompassing Amino Acids: 32-505

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Assay Conditions: Assay done using ELISA technology. 50 ul reaction mix containing HEPES-based buffer and 0-1500 ng FTO is added to the wells coated with the substrate. Incubate overnight at RT. Add antibody against N6-methyladenosine, incubate 1 hr. Then, add secondary HRP-labeled antibody and incubate 30 min. Finally, add HRP chemiluminsecent substrates and read luminescence.

Description: Human FTO, also known as Fat mass and obesity-associated protein, ALKBH9; BMIQ14; GDFD; KIAA1752, and alpha-ketoglutarate-dependent dioxygenase, GenBank Accession No. NM_001080432, a.a 32-505 (end), with N-terminal His-Tag and expressed in a Baculovirus infected Sf9 cell expression system. MW = 56 kDa.

Format: Aqueous buffer solution.

Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, 180 mM Imidazole

Genbank: NM_001080432

Storage Stability: At least 6 months at -80°C.

Tags: N-Terminal His-Tag

Uniprot: Q9C0B1

Warnings: Avoid freeze/thaw cycles. Protein may be diluted to ≥100 µg/ml in PBS + glycerol and stored at -80°C.

Biosafety Level: Not applicable (BSL-1)

References: 1. Frayling, T., et al., Science. 2007; 316(5826): 889-894.2. Dina, C., et al., Nature Genetics. 2007; 39(6): 724-726.

• Artikelnummer: BPS79480
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 79480
• Green Labware: Nein
• Verpackungseinheit: 20 µg
• Mengeneinheit: STK