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G9a (EHMT2), GST-tag (Sf9-derived) Recombinant
G9a (EHMT2), GST-tag (Sf9-derived) Recombinant
Artikelnummer
BPS51001
Verpackungseinheit
20 µg
Hersteller
BPS Bioscience
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Encompassing Amino Acids: 785-1210
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: 50 µlreaction mix (50 mM TRIS pH 8.8, 5mM MgCl2,4mM DTT, 0.05% Tween-20, 40 µM S-adenosylmethionine, and G9a (0.05 - 0.5 ng)add to the wells coated with the substrate on aGBioscience Neutravidin black plate. Incubatefor 1 hr. Add antibody against methylated K9residue of histone H3, incubate 1 hr. Then, addsecondary HRP-labeled antibody and incubate30 min. Finally, add HRP chemiluminescentsubstrates and read luminescence.
Background: EHMT2 (G9a) is a lysine-preferring histone methyltransferase. EHMT2 can methylate itself at K165 and this automethylation is necessary and sufficient to mediate in vivo interaction of EHMT2 with heterochromatin protein-1 (HP1). EHMT2 is responsible for the majority of dimethylation of histone H3 at lysine 9 (H3K9me2) and is required for the efficient repression of developmentally regulated genes during embryonic stem cell differentiation. EHMT2 is overexpressed in various cancers and can can specifically methylate p53 at Lys(373). EHMT2 polymorphisms have been associated with colorectal cancer.
Description: Human G9a, also known as EHMT2 (euchromatic histone-lysine N-methyltransferase 2) and KMT1C (GenBank Accession No. NM_006709), a.a. 785-1210, with N-terminal GST-tag, MW = 74.6 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 Mm KCl, 3 mM DTT and 10% glycerol
Genbank: NM_006709
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: Q96KQ7
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Yuan,X. et al., Mol. Cell 27, 4, 585-595, 2007.
2. Nishida,M. et al., Genes Cells 12, 7, 877-888, 2007. Application Reference(s):
On the histone lysine methyltransferase activity of fungal metabolite chaetocin (2013)
Encompassing Amino Acids: 785-1210
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: 50 µlreaction mix (50 mM TRIS pH 8.8, 5mM MgCl2,4mM DTT, 0.05% Tween-20, 40 µM S-adenosylmethionine, and G9a (0.05 - 0.5 ng)add to the wells coated with the substrate on aGBioscience Neutravidin black plate. Incubatefor 1 hr. Add antibody against methylated K9residue of histone H3, incubate 1 hr. Then, addsecondary HRP-labeled antibody and incubate30 min. Finally, add HRP chemiluminescentsubstrates and read luminescence.
Background: EHMT2 (G9a) is a lysine-preferring histone methyltransferase. EHMT2 can methylate itself at K165 and this automethylation is necessary and sufficient to mediate in vivo interaction of EHMT2 with heterochromatin protein-1 (HP1). EHMT2 is responsible for the majority of dimethylation of histone H3 at lysine 9 (H3K9me2) and is required for the efficient repression of developmentally regulated genes during embryonic stem cell differentiation. EHMT2 is overexpressed in various cancers and can can specifically methylate p53 at Lys(373). EHMT2 polymorphisms have been associated with colorectal cancer.
Description: Human G9a, also known as EHMT2 (euchromatic histone-lysine N-methyltransferase 2) and KMT1C (GenBank Accession No. NM_006709), a.a. 785-1210, with N-terminal GST-tag, MW = 74.6 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 Mm KCl, 3 mM DTT and 10% glycerol
Genbank: NM_006709
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: Q96KQ7
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Yuan,X. et al., Mol. Cell 27, 4, 585-595, 2007.
2. Nishida,M. et al., Genes Cells 12, 7, 877-888, 2007. Application Reference(s):
On the histone lysine methyltransferase activity of fungal metabolite chaetocin (2013)
| Artikelnummer | BPS51001 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 51001 |
| Green Labware | Nein |
| Verpackungseinheit | 20 µg |
| Mengeneinheit | STK |
| Wirt | Insect |
| Produktinformation (PDF) | Download |
| MSDS (PDF) |
|
Immer für Sie da
Gernot Ensinger, M.Sc.
Laurent Haze
