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G9a Homogeneous Assay Kit
G9a Homogeneous Assay Kit
Artikelnummer
BPS52051
Verpackungseinheit
384 rxns.
Hersteller
BPS Bioscience
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Applications: Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Background: G9a is a histone methyltransferase that exhibits methylation activity toward H3-K9.
Contraindications: Avoid green and blue dyes that absorb light in the Alphascreen® signal emission range (520-620 nm), such as Trypan Blue. Avoid the use of the potent singlet oxygen quenchers such as sodium azide (NaN3) or metal ions (Fe2+, Fe3+, Cu2+, Zn2+ and Ni2+). The presence of >1% RPMI 1640 culture medium leads to a signal reduction due to the presence of excess biotin and iron in this medium. MEM, which lacks these components, does not affect Alphascreen® assays.
Description: The G9a Homogeneous Assay Kit is designed to measure the activity of G9a (also known as EHMT2 or KMT1C) for screening and profiling applications, using AlphaLISA® technology. The key to the G9a Homogeneous AssayKit is a highly specific antibody that recognizes methylated substrate. With this kit,only three simple steps on a microtiter plate are required for methyltransferasedetection. First, a sample containing G9a enzyme is incubated with the biotinylatedsubstrate for one hour. Next, acceptor beads and primary antibody are added, thendonor beads, followed by reading the Alpha-counts.
Storage Stability: 12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Supplied As: Kit comes in a convenient AlphaLISA® format, with biotinylated histone H3 peptide substrate, primary antibody, methylation assay buffer, and purified G9a for 384 enzyme reactions.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: Dillon S.C., et al. 2005. Genome Biology 6:227.
Applications: Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Background: G9a is a histone methyltransferase that exhibits methylation activity toward H3-K9.
Contraindications: Avoid green and blue dyes that absorb light in the Alphascreen® signal emission range (520-620 nm), such as Trypan Blue. Avoid the use of the potent singlet oxygen quenchers such as sodium azide (NaN3) or metal ions (Fe2+, Fe3+, Cu2+, Zn2+ and Ni2+). The presence of >1% RPMI 1640 culture medium leads to a signal reduction due to the presence of excess biotin and iron in this medium. MEM, which lacks these components, does not affect Alphascreen® assays.
Description: The G9a Homogeneous Assay Kit is designed to measure the activity of G9a (also known as EHMT2 or KMT1C) for screening and profiling applications, using AlphaLISA® technology. The key to the G9a Homogeneous AssayKit is a highly specific antibody that recognizes methylated substrate. With this kit,only three simple steps on a microtiter plate are required for methyltransferasedetection. First, a sample containing G9a enzyme is incubated with the biotinylatedsubstrate for one hour. Next, acceptor beads and primary antibody are added, thendonor beads, followed by reading the Alpha-counts.
Storage Stability: 12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Supplied As: Kit comes in a convenient AlphaLISA® format, with biotinylated histone H3 peptide substrate, primary antibody, methylation assay buffer, and purified G9a for 384 enzyme reactions.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: Dillon S.C., et al. 2005. Genome Biology 6:227.
| Artikelnummer | BPS52051 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 52051 |
| Green Labware | Nein |
| Verpackungseinheit | 384 rxns. |
| Mengeneinheit | PAK |
| Produktinformation (PDF) | Download |
| MSDS (PDF) |
|
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Gernot Ensinger, M.Sc.
Laurent Haze
